Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene.
Identifieur interne : 000241 ( Main/Exploration ); précédent : 000240; suivant : 000242Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene.
Auteurs : Homa Hajjaran [Iran] ; Mehdi Mohebali [Iran] ; Aref Teimouri [Iran] ; Mohammad Ali Oshaghi [Iran] ; Hamed Mirjalali [Iran] ; Elham Kazemi-Rad [Iran] ; Mohammad Reza Shiee [Iran] ; Saied Reza Naddaf [Iran]Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2014.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Animaux (MeSH), Chiens (MeSH), Données de séquences moléculaires (MeSH), Géographie (MeSH), Humains (MeSH), Iran (épidémiologie), Leishmania (classification), Leishmania (génétique), Leishmaniose cutanée (parasitologie), Leishmaniose cutanée (épidémiologie), Leishmaniose viscérale (parasitologie), Leishmaniose viscérale (épidémiologie), Phylogenèse (MeSH), Phylogéographie (MeSH), Polymorphisme de restriction (MeSH), Rodentia (MeSH), Transferases (other substituted phosphate groups) (génétique).
- MESH :
- génétique : Leishmania, Transferases (other substituted phosphate groups).
- parasitologie : Leishmaniose cutanée, Leishmaniose viscérale.
- épidémiologie : Iran, Leishmaniose cutanée, Leishmaniose viscérale.
- Analyse de séquence d'ADN, Animaux, Chiens, Données de séquences moléculaires, Géographie, Humains, Phylogenèse, Phylogéographie, Polymorphisme de restriction, Rodentia.
English descriptors
- KwdEn :
- Animals (MeSH), Dogs (MeSH), Geography (MeSH), Humans (MeSH), Iran (epidemiology), Leishmania (classification), Leishmania (genetics), Leishmaniasis, Cutaneous (epidemiology), Leishmaniasis, Cutaneous (parasitology), Leishmaniasis, Visceral (epidemiology), Leishmaniasis, Visceral (parasitology), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Phylogeography (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Rodentia (MeSH), Sequence Analysis, DNA (MeSH), Transferases (Other Substituted Phosphate Groups) (genetics).
- MESH :
- chemical , genetics : Transferases (Other Substituted Phosphate Groups).
- geographic , epidemiology : Iran.
- classification : Leishmania.
- epidemiology : Leishmaniasis, Cutaneous, Leishmaniasis, Visceral.
- genetics : Leishmania.
- parasitology : Leishmaniasis, Cutaneous, Leishmaniasis, Visceral.
- Animals, Dogs, Geography, Humans, Molecular Sequence Data, Phylogeny, Phylogeography, Polymorphism, Restriction Fragment Length, Rodentia, Sequence Analysis, DNA.
Abstract
The identity of Iranian Leishmania species has been resolved to some extent by some genetic markers. In this study, based on N-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. DNAs of 121 isolates belonging to cutaneous leishmaniasis (CL) patients, canine visceral leishmaniasis (CVL) cases, and Rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. All the amplicons were analyzed with restriction fragment length polymorphism (RFLP) using Acc1 enzyme, and 49 amplicons representing different reservoir hosts were sequenced and aligned with similar sequences from GenBank database. The RFLP analysis revealed that 41 CL patients were infected Leishmania tropica and 36 with Leishmania major. Among 10 CVL isolates, 6 were identified as Leishmania infantum and 4 as L. tropica. Amongst 34 rodents' isolates, 11 and 23 isolates exhibited patterns similar to those of L. major, and L. tropica/Leishmania turanica, respectively. The sequencing results from all CL patients, CVL cases, and 4 reservoir rodents were in agreement with RFLP analysis and showed 99-100% homologies with the registered species of L. major, L. tropica, and L. infantum from Turkey, Tunisia, Iraq and Israel. Of the 7 rodent isolates exhibiting RFLP patterns similar to L. tropica/L. turanica, 3 exhibited the highest homologies (99-100%) with L. turanica and 4 with Leishmania gerbilli. The 49 nagt DNA sequences were grouped into five clusters representing L. major, L. tropica, L. infantum, L. turanica and L. gerbilli species, encompassing 19 haplotypes. No correlation was observed between intraspecies divergence and geographic distribution of haplotypes. The L. tropica haplotypes exhibited more homologies with those of L. infantum than L. major (97.2% vs. 96.9%), a probable indication to the potential ability of L. tropica to visceralize. Characterization of Iranian Leishmania isolates using nagt gene allowed unambiguous identification of five prevalent species with a high-resolution phylogeny.
DOI: 10.1016/j.meegid.2014.05.026
PubMed: 24911282
Affiliations:
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Electronic address: hajaranh@tums.ac.ir.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mohebali, Mehdi" sort="Mohebali, Mehdi" uniqKey="Mohebali M" first="Mehdi" last="Mohebali">Mehdi Mohebali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Teimouri, Aref" sort="Teimouri, Aref" uniqKey="Teimouri A" first="Aref" last="Teimouri">Aref Teimouri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Oshaghi, Mohammad Ali" sort="Oshaghi, Mohammad Ali" uniqKey="Oshaghi M" first="Mohammad Ali" last="Oshaghi">Mohammad Ali Oshaghi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mirjalali, Hamed" sort="Mirjalali, Hamed" uniqKey="Mirjalali H" first="Hamed" last="Mirjalali">Hamed Mirjalali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Kazemi Rad, Elham" sort="Kazemi Rad, Elham" uniqKey="Kazemi Rad E" first="Elham" last="Kazemi-Rad">Elham Kazemi-Rad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Shiee, Mohammad Reza" sort="Shiee, Mohammad Reza" uniqKey="Shiee M" first="Mohammad Reza" last="Shiee">Mohammad Reza Shiee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Naddaf, Saied Reza" sort="Naddaf, Saied Reza" uniqKey="Naddaf S" first="Saied Reza" last="Naddaf">Saied Reza Naddaf</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: snaddaf_2001@yahoo.com.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Parasitology, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24911282</idno><idno type="pmid">24911282</idno><idno type="doi">10.1016/j.meegid.2014.05.026</idno><idno type="wicri:Area/PubMed/Corpus">000573</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000573</idno><idno type="wicri:Area/PubMed/Curation">000570</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000570</idno><idno type="wicri:Area/PubMed/Checkpoint">000576</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000576</idno><idno type="wicri:Area/Main/Merge">000241</idno><idno type="wicri:Area/Main/Curation">000241</idno><idno type="wicri:Area/Main/Exploration">000241</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene.</title><author><name sortKey="Hajjaran, Homa" sort="Hajjaran, Homa" uniqKey="Hajjaran H" first="Homa" last="Hajjaran">Homa Hajjaran</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: hajaranh@tums.ac.ir.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mohebali, Mehdi" sort="Mohebali, Mehdi" uniqKey="Mohebali M" first="Mehdi" last="Mohebali">Mehdi Mohebali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Teimouri, Aref" sort="Teimouri, Aref" uniqKey="Teimouri A" first="Aref" last="Teimouri">Aref Teimouri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Oshaghi, Mohammad Ali" sort="Oshaghi, Mohammad Ali" uniqKey="Oshaghi M" first="Mohammad Ali" last="Oshaghi">Mohammad Ali Oshaghi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mirjalali, Hamed" sort="Mirjalali, Hamed" uniqKey="Mirjalali H" first="Hamed" last="Mirjalali">Hamed Mirjalali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Kazemi Rad, Elham" sort="Kazemi Rad, Elham" uniqKey="Kazemi Rad E" first="Elham" last="Kazemi-Rad">Elham Kazemi-Rad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Shiee, Mohammad Reza" sort="Shiee, Mohammad Reza" uniqKey="Shiee M" first="Mohammad Reza" last="Shiee">Mohammad Reza Shiee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Naddaf, Saied Reza" sort="Naddaf, Saied Reza" uniqKey="Naddaf S" first="Saied Reza" last="Naddaf">Saied Reza Naddaf</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: snaddaf_2001@yahoo.com.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Parasitology, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Dogs (MeSH)</term><term>Geography (MeSH)</term><term>Humans (MeSH)</term><term>Iran (epidemiology)</term><term>Leishmania (classification)</term><term>Leishmania (genetics)</term><term>Leishmaniasis, Cutaneous (epidemiology)</term><term>Leishmaniasis, Cutaneous (parasitology)</term><term>Leishmaniasis, Visceral (epidemiology)</term><term>Leishmaniasis, Visceral (parasitology)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phylogeography (MeSH)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Rodentia (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Transferases (Other Substituted Phosphate Groups) (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Animaux (MeSH)</term><term>Chiens (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Géographie (MeSH)</term><term>Humains (MeSH)</term><term>Iran (épidémiologie)</term><term>Leishmania (classification)</term><term>Leishmania (génétique)</term><term>Leishmaniose cutanée (parasitologie)</term><term>Leishmaniose cutanée (épidémiologie)</term><term>Leishmaniose viscérale (parasitologie)</term><term>Leishmaniose viscérale (épidémiologie)</term><term>Phylogenèse (MeSH)</term><term>Phylogéographie (MeSH)</term><term>Polymorphisme de restriction (MeSH)</term><term>Rodentia (MeSH)</term><term>Transferases (other substituted phosphate groups) (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transferases (Other Substituted Phosphate Groups)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Iran</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Leishmania</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Leishmaniasis, Cutaneous</term><term>Leishmaniasis, Visceral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Leishmania</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Leishmania</term><term>Transferases (other substituted phosphate groups)</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Leishmaniose cutanée</term><term>Leishmaniose viscérale</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Leishmaniasis, Cutaneous</term><term>Leishmaniasis, Visceral</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Iran</term><term>Leishmaniose cutanée</term><term>Leishmaniose viscérale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dogs</term><term>Geography</term><term>Humans</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Phylogeography</term><term>Polymorphism, Restriction Fragment Length</term><term>Rodentia</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Chiens</term><term>Données de séquences moléculaires</term><term>Géographie</term><term>Humains</term><term>Phylogenèse</term><term>Phylogéographie</term><term>Polymorphisme de restriction</term><term>Rodentia</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The identity of Iranian Leishmania species has been resolved to some extent by some genetic markers. In this study, based on N-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. DNAs of 121 isolates belonging to cutaneous leishmaniasis (CL) patients, canine visceral leishmaniasis (CVL) cases, and Rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. All the amplicons were analyzed with restriction fragment length polymorphism (RFLP) using Acc1 enzyme, and 49 amplicons representing different reservoir hosts were sequenced and aligned with similar sequences from GenBank database. The RFLP analysis revealed that 41 CL patients were infected Leishmania tropica and 36 with Leishmania major. Among 10 CVL isolates, 6 were identified as Leishmania infantum and 4 as L. tropica. Amongst 34 rodents' isolates, 11 and 23 isolates exhibited patterns similar to those of L. major, and L. tropica/Leishmania turanica, respectively. The sequencing results from all CL patients, CVL cases, and 4 reservoir rodents were in agreement with RFLP analysis and showed 99-100% homologies with the registered species of L. major, L. tropica, and L. infantum from Turkey, Tunisia, Iraq and Israel. Of the 7 rodent isolates exhibiting RFLP patterns similar to L. tropica/L. turanica, 3 exhibited the highest homologies (99-100%) with L. turanica and 4 with Leishmania gerbilli. The 49 nagt DNA sequences were grouped into five clusters representing L. major, L. tropica, L. infantum, L. turanica and L. gerbilli species, encompassing 19 haplotypes. No correlation was observed between intraspecies divergence and geographic distribution of haplotypes. The L. tropica haplotypes exhibited more homologies with those of L. infantum than L. major (97.2% vs. 96.9%), a probable indication to the potential ability of L. tropica to visceralize. Characterization of Iranian Leishmania isolates using nagt gene allowed unambiguous identification of five prevalent species with a high-resolution phylogeny. </div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><country name="Iran"><noRegion><name sortKey="Hajjaran, Homa" sort="Hajjaran, Homa" uniqKey="Hajjaran H" first="Homa" last="Hajjaran">Homa Hajjaran</name></noRegion><name sortKey="Kazemi Rad, Elham" sort="Kazemi Rad, Elham" uniqKey="Kazemi Rad E" first="Elham" last="Kazemi-Rad">Elham Kazemi-Rad</name><name sortKey="Mirjalali, Hamed" sort="Mirjalali, Hamed" uniqKey="Mirjalali H" first="Hamed" last="Mirjalali">Hamed Mirjalali</name><name sortKey="Mohebali, Mehdi" sort="Mohebali, Mehdi" uniqKey="Mohebali M" first="Mehdi" last="Mohebali">Mehdi Mohebali</name><name sortKey="Naddaf, Saied Reza" sort="Naddaf, Saied Reza" uniqKey="Naddaf S" first="Saied Reza" last="Naddaf">Saied Reza Naddaf</name><name sortKey="Oshaghi, Mohammad Ali" sort="Oshaghi, Mohammad Ali" uniqKey="Oshaghi M" first="Mohammad Ali" last="Oshaghi">Mohammad Ali Oshaghi</name><name sortKey="Shiee, Mohammad Reza" sort="Shiee, Mohammad Reza" uniqKey="Shiee M" first="Mohammad Reza" last="Shiee">Mohammad Reza Shiee</name><name sortKey="Teimouri, Aref" sort="Teimouri, Aref" uniqKey="Teimouri A" first="Aref" last="Teimouri">Aref Teimouri</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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